BIOCHAGA (CHAGA, INONOTUS OBLIQUUS) SUVLI EKSTRAKTINING SICHQON KOLOREKTAL KARSINOMASI (CT26) VA FIBROBLAST (L929) HUJAYRA LINIYALARIGA IN VITRO SITOTOKSIK TA'SIRI
Keywords:
in vitro, L929, CT26, tripan ko‘k, sitotoksiklik, Inonotus obliquus, chagaAbstract
Maqolada chaga (Inonotus obliquus) suvli ekstrakti («BioChaga») ning sichqon kolorektal karsinomasi CT-26 va normal fibroblastsimon L929 hujayra liniyalariga in vitro sitotoksik hamda antiproliferativ ta'siri tripan ko‘k bilan bo‘yash usulida (1,10,100 mkg/ml, 48 soat) o‘rganilgan. Ekstrakt ikkala liniyada ham doza-bog‘liq ta'sir ko‘rsatdi. 100 mkg/ml konsentratsiyada CT-26 hujayralarida o‘lik hujayralar ulushi taxminan 33 % ga yetdi, L-929 da esa 3 % dan oshmadi, biroq tirik hujayralar ulushi kamaydi; bu ekstraktning sitostatik (antiproliferativ) ta'siriga ishora qilishi mumkin. Natijalar ekstraktning o‘sma hujayralariga nisbatan qisman selektivligini ko‘rsatadi, uni tasdiqlash uchun qo‘shimcha tadqiqotlar talab etiladi.
References
1. Newman D.J., Cragg G.M. Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019. Journal of Natural Products. 2020;83(3):770-803. DOI: 10.1021/acs.jnatprod.9b01285.
DOI: 10.1021/acs.jnatprod.9b01285
2. Kamiloglu S., Sari G., Ozdal T., Capanoglu E. Guidelines for cell viability assays. Food Frontiers. 2020;1(3):332–349. DOI: 10.1002/fft2.44.
DOI: 10.1002/fft2.44
3. Shashkina M.Y., Shashkin P.N., Sergeev A.V. Chemical and medicobiological properties of Chaga (review). Pharmaceutical Chemistry Journal. 2006;40(10):560–568. DOI: 10.1007/s11094-006-0194-4.
DOI: 10.1007/s11094-006-0194-4
4. Kim J., Yang S.C., Hwang A.Y., et al. Composition of triterpenoids in Inonotus obliquus and their anti-proliferative activity on cancer cell lines. Molecules. 2020;25(18):4066. DOI: 10.3390/molecules25184066.
DOI: 10.3390/molecules25184066
5. Youn M.J., Kim J.K., Park S.Y., et al. Potential anticancer properties of the water extract of Inonotus obliquus by induction of apoptosis in melanoma B16-F10 cells. Journal of Ethnopharmacology. 2009;121(2):221–228. DOI: 10.1016/j.jep.2008.10.016.
DOI: 10.1016/j.jep.2008.10.016
6. Lee S.H., Hwang H.S., Yun J.W. Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT-29 human colon cancer cells. Phytotherapy Research. 2009;23(12):1784–1789. DOI: 10.1002/ptr.2836.
DOI: 10.1002/ptr.2836
7. Strober W. Trypan Blue Exclusion Test of Cell Viability. Current Protocols in Immunology. 2015;111:A3.B.1–A3.B.3. DOI: 10.1002/0471142735.ima03bs111.
DOI: 10.1002/0471142735.ima03bs111
8. Mishra SK, Kang JH, Song KH, Park MS, Kim DK, Park YJ, et al. Inonotus obliquus suppresses proliferation of colorectal cancer cells and tumor growth in mice models by downregulation of β-catenin/NF-κB-signaling pathways. Eur J Inflamm. 2013;11(3). https://doi.org/10.1177/1721727X1301100306
9. Kang JH, Jang JE, Mishra SK, Lee HJ, Nho CW, Shin D, et al. Ergosterol peroxide from Chaga mushroom (Inonotus obliquus) exhibits anti-cancer activity by down-regulation of the β-catenin pathway in colorectal cancer. J Ethnopharmacol. 2015;173:303–312. https://doi.org/10.1016/j.jep.2015.07.030
10. Arata S, Watanabe J, Maeda M, Yamamoto M, Matsuhashi H, Mochizuki M, et al. Continuous intake of the Chaga mushroom (Inonotus obliquus) aqueous extract suppresses cancer progression and maintains body temperature in mice. Heliyon. 2016;2(5):e00111. https://doi.org/10.1016/j.heliyon.2016.e00111
